What is 16S rRNA sequencing?
16S rRNA gene sequencing is commonly used for identification, classification and quantitation of microbes within complex biological mixtures such as environmental samples (example marine water) and gut samples (example human gut microbiome). Conveniently, the 16S rRNA gene consists of both conserved and variable regions
Why is the 16S rRNA sequence used for identification?
Because of the complexity of DNA–DNA hybridization, 16S rRNA gene sequencing is used as a tool to identify bacteria at the species level and assist with differentiating between closely related bacterial species. Many clinical laboratories rely on this method to identify unknown pathogenic strains.
Some of the key deliverables for 16s rRNA data analyses are:
– Sequencing QC
– Reads Classification
– Taxonomical Abundance
– Taxa Abundance Heatmap
– Sankey Diagram
– Venn Diagram
– Alpha dan Beta-Diversity Analysis
Genetika Science Indonesia is offering 16S analysis using real-time, long-read nanopore sequencing
The 16S rRNA gene is present in all bacteria and archaea. The gene is ideal for sequence-based identification of these organisms, particularly in mixed samples, due to the presence of conserved and highly variable regions. By narrowing down to a specific region of interest, all the organisms present in the sample can be seen without sequencing unnecessary regions of the genome, making the analysis quicker and more economical.