Genetika Science Indonesia is offering RNA-Seq service as one of our portfolio service parts.
Transcriptome sequencing (RNA-Sequencing) is the set of genes which are transcribed in any one condition is known as the transcriptome, and the process of determining the genetic codes contained in the transcriptome, and their relative proportions, is known as transcriptome sequencing.
A transcriptome is the full range of messenger RNA, or mRNA, molecules expressed by an organism. The term “transcriptome” can also be used to describe the array of mRNA transcripts produced in a particular cell or tissue type.
What can a transcriptome tell us?
An RNA sequence mirrors the sequence of the DNA from which it was transcribed. Consequently, by analyzing the entire collection of RNA sequences in a cell (the transcriptome) researchers can determine when and where each gene is turned on or off in the cells and tissues of an organism.
What kind of RNA-Seq services that Genetika Science offer?
- mRNA Sequencing
- Prokaryote RNA Sequencing
- Whole-Transcriptome Sequencing
Sample Requirements:
1) Total RNA samples, please be sure for the requirements:
a) Concentration is ≥ 50 ng/uL.
b) RNA Integrity Number is ≥ 6.5, smooth base line.
c) Purity:
OD260/280≥ 2.0
OD260/230 ≥ 2.0
2) Samples such as plants tissue, animal tissue, pure bacterial isolate, etc.
Send the samples dry ice condition or liquid nitrogen.
What is the platform that Genetika Science will run?
Illumina NextSeq 500 PE150
What Will You Get From Genetika Lab?
- Alignment.
- DEG.
- Heatmap.
- MA-Plot.
- Volcano Plot.
- Gene ontology.
- SNP, InDel.
- Venn Diagrams.