SARS-CoV-2 Spike S1-RBD IgG&IgM ELISA Detection Kit
The GenScript SARS-CoV-2 Spike S1-RBD IgG&IgM ELISA Detection Kit is intended for the determination of IgG and IgM antibodies separately against SARS-CoV-2 Spike S1-Receptor Binding Domain (RBD) in human serum or plasma.
The GenScript SARS-CoV-2 Spike S1-RBD IgG&IgM ELISA Detection Kit is an indirect ELISA detection tool, which can be used for evaluation of anti-SARS-CoV-2 Spike S1-RBD IgG or IgM in human samples. A purified recombinant SARS-CoV-2 Spike S1-RBD antigen is bound to the wells of a Capture Plate. A horseradish peroxidase (HRP) coupled anti-human IgG conjugate is used for IgG determination. Similarly, a HRP conjugated mouse anti-Human IgM is used for IgM determination.
When the Positive control, Negative Control, and specimen are added to capture plates, the positive control and SARS-CoV-2 spike protein S1-RBD antibodies in specimen can be captured on the plate. Other unbound molecules are removed by the washing steps. Then, the HRP conjugated Mouse anti-Human IgG or HRP conjugated mouse anti-human IgM is added to the plate. After washing steps, TMB solution is added and the color turns blue. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a microtiter plate reader. The absorbance of the sample dependents on the titer of the anti-S1-RBD protein antibodies.
Intra-assay: One known level of control was spiked into sample buffer as a test sample. The sample was tested 10 times on the same plate to evaluate intra-assay precision of the kit. Intra-assay variation of this kit is less than or equal to 10%.
Inter-assay: One known level of control was spiked into sample buffer as a test sample. The sample was tested on 3 plates which were randomly selected from 3 different lots to evaluate inter-assay precision of the kit. Inter-assay variation of this kit is less than or equal to 15%.